An Unbiased View of how HPLC works
An Unbiased View of how HPLC works
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ディテクター（検出器）としては目的とする物質の性質に応じて光学的性質（吸光度、屈折率、蛍光等）、電気化学的性質、質量分析法などを利用する装置がある。
각각 다른 산업 분야에 대한 자세한 정보 및 다양한 카테고리는 다음 써모 피셔 사이언티픽 학습 센터에서 산업 및 응용 과학 페이지를 확인하세요.
Column troubles: A soiled or ruined column can cause peak broadening. Contaminants can accumulate to the column after a while, hindering analyte separation. Often cleanse the column based on the company's Guidelines. If cleansing doesn't help, consider replacing the column.
Compatibility: The solvent shouldn't react Together with the analytes or degrade the sample matrix. Check with basic safety details sheets (SDS) for compatibility facts.
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カラム周辺の温度の変動によって溶出時間が安定せず再現性が悪くなる場合があるため、カラム温度を一定に保つために使用する。またカラム温度を分離条件のパラメーターの一つとして積極的に利用する場合もある。
Info analysis software program is essential for interpreting the data acquired within the detector. The software displays the chromatogram, that is a plot of detector sign versus time. Key details points contain:
The pump is the guts of your HPLC system. It provides the cell phase at a relentless and high force (around four hundred atm) through more info the column. Regular move charge is essential for reaching optimum separation and sustaining reproducibility. Aspects to consider when deciding on a move level include things like:
The get of elution of compounds with the column is ruled via the intensity of contact with the stationary stage. The eluent Along with the divided chemical compounds flows previous the detector.
Retention instances: Time it will take for every analyte to get to the detector, delivering a characteristic fingerprint for identification.
, such as, demonstrates retention occasions for four weak acids in two cellular phases with virtually identical values for (P^ key ). Although the purchase of elution is identical for both equally cellular phases, Every single solute’s retention time is affected in different ways by the choice of natural solvent.
If the answer is diluted the region of the peak are going to be considerably less, though the detention time will be exact. Hence it is feasible to detect check here a compound existing even in an exceedingly modest quantity.
검토 중에서 컬럼이나 이동상 등 여러 조건의 조합은 분석 가능성의 큰 영향을 미칩니다.)
The selection to start with acetonitrile is arbitrary—we can easily just as effortlessly decide on to start with methanol or with tetrahydrofuran.